Le ndlela, nangona ixhaphake, inokungabikho kwemingcipheko. Yiyo le nto eyenza iingcali zifune ukujonga iindlela ezintsha ezivumela ukugcina i-ejaculate ixesha elide. I-disadvantage eyona ndlela yendlela yokwenza i-cryopreservation ekhankanywe ngasentla ingabizwa ngokuba yinto yokuba emva kokupheliswa ngokupheleleyo kwesidoda, ukuhamba kweeseli zesini eziqulethwe kuyo kunciphisa malunga nama-20-25%. Oku kuthetha ukuba amathuba okukhulelwa ngexesha lokucwiliswa kweqanda elivuthiweyo elinjalo le-spermatozoa liyancipha.
Xa uphatha umlanjana ngale ndlela, ukugcina umdaka kuthatha ubushushu be -196 degrees.
Indlela yokugcinwa kwesilisa nge teknoloji K. Saito
Le ndlela yokulondoloza i-ejaculate yindoda ingasetyenzwa kula maxesha xa kungekho mfuneko yokulinda ixesha elide le-IVF. Akubandakanyi ukunqumla kwesidoda.
Xa usebenzisa le ndlela, izimo zokugcina izityalo zithatha ukusetyenziswa kwendawo ebizwa ngokuba yi-electrolyte-free (BES). Ngaloo ndlela, isisombululo esingenaso ityuwa, isisombululo esisisigxina se-glucose isetyenziswa rhoqo. Kubalulekile ukuba uqaphele ukuba kude kube sekupheleni kwendlela yokugcina ukusetyenziswa kweeseli zesini zesini kwisisombululo asizange sifunde. Nangona kunjalo, uphando oluninzi luye lwabonisa ukuba iziqulatho ze-sodium ne-potassium zivaliwe ebanda, ezingabandakanywa xa i-isotonic glucose isetyenziswa. Ngamazwi alula, ukusetyenziswa kwezi zisombululo zokugcina i-ejaculate yamadoda kuvumela ukulondoloza i-spermatozoa ngaphandle kweqhwa, ngaphandle kokutshintsha iimpawu zabo zokuziphatha.
Le ndlela ayivumeli ukugcina imbewu yesibindi ixesha elide ngelixa le-cryopreservation. Yingakho ingasetyenziselwa, umzekelo, xa uthatha i-biomaterials iintsuku ezimbalwa ngaphambi kwe-IVF okanye xa kufuneka ukuchumisa okulandelayo ukuba le yangaphambili iphumelele.
Ingakanani imbewu engagcinwa?
Olu hlobo lombuzo lulona lunomdla kulawo madoda angakulungeli kulo mzuzu ukuba abe ooyise.
Okokuqala, kufuneka kuqatshelwe ukuba yonke into ixhomekeke ekukhetheni kwindlela yokugcina i-ejaculate yesilisa. Phantse zonke iibhanki zamabhinqa zamanje zisebenzisa indlela yokwenza i-cryopreservation. Ikuvumela ukuba ugcine i-ejaculate ixesha elide - ukuya kwiminyaka emininzi.
Xa usebenzisa i-technique engabandakanyi ukukhenkcezwa kwe-ejaculate, ayigcinwanga ngaphezu kweenyanga 1. Njengoko sele kukhankanywe ngasentla, le ndlela isetyenziswe ikakhulu kwinkqubo ye- in vitro fertilization.
Nangona kunjalo, ixesha elincinane lokugcinwa kweesilwanyana alinakuchaphazela i-motility ye-spermatozoa.
I-ejaculate igcinwa njani?
Leyo nkqubo yokukhethwa kwesidoda iqhutyelwa kwiziko lempilo elithile. Indoda inikwe i-flask eyinyumba, apho i-ejaculate iqokelelwa yi-masturbation.
Emva koko, i-flask ngokwayo ifakwe kwisixhobo esikhethekileyo, i-reagent apho i-nitrogen eninzi ivela khona, kwaye ivaliwe ngokuqinile.
Xa kudingekile, i-sperm flask isuswe kwaye ichithwe. Emva koko, umgangatho wokulondolozwa kwawo uvavanywa ngokuvavanya isampuli kwi-microscope ekhethekileyo.