I-cryopreservation yesidoda

Iyeza lokuzala likhula ngezinga elimangalisayo, kwaye ngoku abaninzi abantu abatshatileyo abaya kufumana isigwebo "sokungabikho ntsapho" banethuba lokuba ngabazali. Ukuchithwa kwe-spropervation of sperm yintsebenzo enjalo eyenziwa kakhulu kwiinkqubo zobuchwepheshe bokuzala (ART). Siza kuqhelana nezibonakaliso ze-cryopreservation yembewu kunye neziganeko zobuchwepheshe.

Yiyiphi inqabileyo yesidoda?

Kukho inani leenombolo, ngokubhekiselele kukuthi kwenziwa i-cryopreservation ye-spermatozoa, iquka:

• ukudala ibhanki yesidoda;
• Kwimeko apho kungenakwenzeka ukufumana i-spermatozoa yendoda yokuchumisa i-ovule ngosuku lokuvuthwa kwe-follicle kumfazi;
• ukuba kuyimfuneko ukufumana ingxube eyaneleyo ye-spermatozoa ephezulu, ubuninzi obuninzi kwi-ejaculate (oligozoospermia).

Ukukhupha umdaka kunye namaqanda kukunyathelo eliphambili kumachiza okuzala. Le nkqubo inenkcazo ukuba i-spermatozoa isuswe ngokugqithiseleyo ukuphepha ukuqokelela ngokuphindaphindiweyo kwesidoda. Ukongeza, kwanona mntu ozalisekileyo ngokupheleleyo akakwazi ukukhuselwa zizifo kunye nokulimala okunokunciphisa ikhono lomntu lokukhulelwa okanye, ngokubanzi, alubandakanyi. Yaye ikhupha isidoda sayo, indoda inelungelo lokwenene lokuba nguyise.

Ukulungiselela i-cryopreservation yesidoda

Ngaphambi kokuqhwala isidoda sakho, indoda kufuneka ihlolwe. Ngoko, ukuhlalutya okuyimfuneko kukuba:

• Uviwo lwe-syphilis, igciwane lesifo sokukhusela i-immunodeficiency virus, i-hepatitis B kunye no-C;
• iimvavanyo zesifo socansi.

Kucetyiswa ukuqhuba i-cryotest, oko kukuthi, emva kokuqhwaqhalwa yi-teknoloji ekhethekileyo, ingabonakaliswa nenye i-sperm doner ukuvavanya umgangatho wayo emva kokuphazamiseka nokusebenza kwe-spermatozoa.

Inkqubo yokuqokelela kunye nokulondoloza isibindi

Ukukhupha i-spermatozoa inamanqanaba ambalwa.

1. Ngoko, isinyathelo sokuqala kukufumana umdaka kwaye ugcine kwindawo yokushisa kweyure ukuya kwiyure enye, ukwenzela ukuba ukupheliswa komntu kuvele.
2. Kwinqanaba lesibini, iqhwa ngokwalo lenziwe ngokongeza i-cryoprotectant kwi-ejaculate, ekhusela ukutshatyalaliswa kwe-spermatozoa ngexesha lokuqhaqhaqha, ukukhenkcezwa kwamanzi kwiiseli kwaye kwenza iimbumba zeseli zibe zizinzile.
3. Emva kokungeniswa kwe-cryoprotectant, ukubunjwa okubangelwa kukushiya imizuzu engama-15, emva koko bazaliswe izitya ezikhethekileyo (i-cryosolomines). Iibhubhu ezineziyobisi zigcinwe kwikamelo elipholileyo kuphela kwindawo ephakamileyo, kufuneka zimakwe kwaye zivalwe ngokuqinile.

Inkqubo yomngcipheko iqhutyelwa kancane kancane kwiqondo lokushisa -198 ° C (ukushisa kwe-nitrojeni yamanzi). Ukuhluthwa kwesidoda kufuneka kube nangoko ngaphambi kokuba inkqubo ye- vitro fertilization okanye insemination ifakwe.

Kakade, akusiyo yonke i-spermatozoa egcina amandla abo okuchumisa ngexesha elifanelekileyo, kodwa ngokulinganiselwa kuma-75% ahlala epheleleyo, kwaye oku kunele ngokwaneleyo ukuchumisa ngempumelelo. Impumelelo yokukhulelwa emva kokuchumisa (insemination okanye i-IVF) kunye nesidoda esahlambulukileyo kwaye esandul 'phantse ifana ngokufanayo.

Ngoko, inkqubo yokwenza i-cryopreservation ye-sperm yenye yezobuchwepheshe obutsha kakhulu zonyango lwangoku, apho ezininzi izibini kunye namadoda amancinci anika ithemba lokuzalwa komntwana. Iphuzu elibi lixabiso layo eliphezulu, njengoko imfuneko yezixhobo ezibiza kakhulu ukukhupha kunye nokugcinwa kwamalungiselelo ngokunyusa kakhulu kwandisa intengo. Kwaye, oku, kwenza ukuba kube lula kubantu abanomyinge kwaye ngaphantsi kwinqanaba eliphakathi kwempumelelo.