I-Cryopreservation ye-embrasi yindlela ebenza bahlale bebenokusebenza iminyaka emininzi kunye nokukwazi ukuhlula emva kokuhlutha. Inkqubo ye- cryopreservation yenziwa ngokunyanga ama-embrosi nge-nitrogen. Kule nqaku siza kuqwalasela iinkalo zendlela yokuqhwabalalisa, iintlobo zabo, kwakunye neenkcukacha ze-embroprotective embryos kwisibilini se-uterine ngexesha lokuchumisa i-vitro .
I-teknoloji ye-embryo epholile
Kukho iindlela ezimbini zokubanda ama-embryythi: i-vitrification kunye ne "slowly frozen". I-teknoloji ye "iphothilizi epholileyo" ibhekwa ingasebenzi kwaye iyeke ukusetyenziswa kwiiklinikhi ezininzi. Iqukethe ukuhambisa ama-embrasi aqriweyo kwi-straw ene-cryoprotectant, kwaye ivelisa amanzi. Ukukhupha umbungu kukukhusela kwiqhwa, kodwa kunokukhokelela ekudambiseni amanzi. Oku kunciphisa inani leembrusi ezingenako kuphela ukuphila, kodwa nokugcina ukukwazi ukwahlula nokuhlukana.
I-Vitrification ye-embrasi yindlela ekhoyo yangoku, equlethe ukuwaphatha nge-nitrojeni yamanzi, ngelixa amanzi aqulethweyo aguqulwa abe yi-jelly.
Izixhobo zokwenza i-cryopreservation zibiza kakhulu, ingakumbi leyo isetyenziselwa "ukucotha ukuphoxisa". I-shelf-ubomi beemibusi ezinqabileyo kufuneka zingadluli iminyaka emi-5.
Ukutshintshwa kweembungu emva kwe-cryopreservation
I-embryo, eyavela kubanda ngaphambi kokuba ifakwe kwisibeletho, kufuneka ilungiselelwe. Ukwenza oku, batyhutyhulwa kwiqondo lokushisa lokushisa kwaye baphathwa ngesisombululo esikhethekileyo esiya kunceda imbryo ukuba igcine izakhiwo zayo. Ukutshintshwa kweembrusi ezifudumeleyo zenziwa ngepathati ekhethekileyo, efakwe kwinqanawa yomlomo wesibeleko kwisibilini se-uterine.
Sihlolisise ubuchwepheshe obukhoyo be-embrying freeze, kunye nenkqubo yokubuyisela iimbumba ezinqabileyo. I-Cryopreservation ye-embrasi yenza kube lula ukufaka kwakhona ukuba akukho khulelwa emva kwenkqubo yokuqala.